Effects of
Lewis Y Oligosaccharide on Secretion and Gene Expression of EGF and EGF-R in
Mouse Embryos
KONG Ying, GE Chang-Hui, LI Hong, ZHU
Zheng-Mei*
( Department of Biochemistry,Institute of Glycobiology,Dalian Medical
University,Dalian 116027,China )
Abstract
Implantation is a complicated developmental process. Some hormones and
cytokines are involved in the regulation of the implantation by regulating
functions of the endometrium and the embryo.Oligosaccharide antigen Lewis Y is
specifically expressed on embryo surface and plays an important role in
implantation.In this study, effects of Lewis Y on secretion and gene expression
of EGF and EGF receptor of the embryo were investigated.Embryos were
pre-incubated with LeY specific antibody AH6 for 3 h,then antibody
was removed, immuno-dot blots and RT-PCR were used to analyze the secretion of
EGF and EGF receptor and their gene expression in the embryo at different times
after pre-incubation.The results showed that, when blocked by AH6 antibody for
1.5 h,the secretion and expression of EGF in the embryo were significantly
declined, and the alteration was continued to over 6 h;and those of EGF-R were
slightly decreased after blocking.The results suggest that Lewis Y
oligosaccharide on the surface of embryo may be involved in the regulation of
embryo development and implantation,by affecting the expression and secretion
of EGF in embryo.
Key words embryo; LeY oligosaccharide
antigen; epidermal growth factor; monoclonal antibody
*Corresponding author£ºTel,86-411-4720616;e-mail, [email protected]